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Paul N Gardner
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ID Biomedical Corporation
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Federation of European Neuroscience Societies
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Promega
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GeNOsys Inc
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GenScript corporation
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Active Motif
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International Federation of Clinical Chemistry and Laboratory Medicine
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B-Bridge Inc
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Ribobio co
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Image Search Results
Journal: Free radical biology & medicine
Article Title: The HIV-Tat protein interacts with Sp3 transcription factor and inhibits its binding to a distal site of the sod2 promoter in human pulmonary artery endothelial cells
doi: 10.1016/j.freeradbiomed.2019.12.015
Figure Lengend Snippet: Using an Sp-specific oligonucleotide-binding ELISA (TransAm, Active Motif), we measured the levels of active Sp1 (Panel A) and Sp3 (Panel B) in the nucleus of mock transfected (Tat(−)) or pCP2-Tat101 transfected (Tat(+)) HPAEC. The signal was normalized to μg of nuclear protein. Mean ± SEM of biological replicates, n = 3, (***p ≤ 0.001 by unpaired student’s T-test).
Article Snippet: DNA-binding ELISAs for active Sp1 and Sp3 were carried out on nuclear extracts prepared as previously described [ 21 ] using Sp1 and Sp3-specific
Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Transfection